CK10/Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody, Clone: [1D8], Unconjugated

Catalog Number: BYT-ORB500076
Article Name: CK10/Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody, Clone: [1D8], Unconjugated
Biozol Catalog Number: BYT-ORB500076
Supplier Catalog Number: orb500076
Alternative Catalog Number: BYT-ORB500076-50,BYT-ORB500076-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthesized peptide derived from human Cytokeratin 10 (550-584aa)
Conjugation: Unconjugated
Alternative Names: BCIE, BIE, CK10, EHK, EHK2, EHK2A, EHK2B, IHL, K10, KPP, D130054E02Rik, K1C1, Krt-1.10, Krt1-10, Ka10, K1C10_HUMAN, KRT10, Cytokeratin-10 (CK-10), Keratin-10 (K10), K1C10_MOUSE, 56 kDa cytokeratin, Keratin, type I cytoskeletal 59 kDa, K1C10_RAT, Type I keratin Ka10,
CK10/Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Clone Designation: [1D8]
Molecular Weight: 59 kDa
UniProt: P13645
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: KRT10
Application Dilute: WB=1:1000-10000, IHC-P=1:1000-5000, IHC-F=1:1000-5000, IF=1:1000-5000
Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CK10) Monoclonal Antibody, Unconjugated (orb500076) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CK10) Monoclonal Antibody, Unconjugated (orb500076) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Skin (Mouse) Lysate at 40 ug, Skin (Rat) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-CK10/Cytokeratin 10 (orb500076) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 63kD.
WB analysis of Skin (Mouse) Lysate at 40 ug, Skin (Rat) Lysate at 40 ug using CK10 antibody
IHC-P image of mouse skin tissue using CK10 antibody
IHC-P image of rat skin tissue using CK10 antibody