CK10/Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody, Clone: [1D8], Unconjugated

Artikelnummer: BYT-ORB500076

Artikelname:	CK10/Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody, Clone: [1D8], Unconjugated
Artikelnummer:	BYT-ORB500076
Hersteller Artikelnummer:	orb500076
Alternativnummer:	BYT-ORB500076-50,BYT-ORB500076-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	A synthesized peptide derived from human Cytokeratin 10 (550-584aa)
Konjugation:	Unconjugated
Alternative Synonym:	BCIE, BIE, CK10, EHK, EHK2, EHK2A, EHK2B, IHL, K10, KPP, D130054E02Rik, K1C1, Krt-1.10, Krt1-10, Ka10, K1C10_HUMAN, KRT10, Cytokeratin-10 (CK-10), Keratin-10 (K10), K1C10_MOUSE, 56 kDa cytokeratin, Keratin, type I cytoskeletal 59 kDa, K1C10_RAT, Type I keratin Ka10,

CK10/Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody

Klonalität:	Recombinant
Konzentration:	1mg/ml
Klon-Bezeichnung:	[1D8]
Molekulargewicht:	59 kDa
UniProt:	P13645
Puffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:	Liquid
Target-Kategorie:	KRT10

Application Verdünnung:

WB=1:1000-10000, IHC-P=1:1000-5000, IHC-F=1:1000-5000, IF=1:1000-5000

	Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CK10) Monoclonal Antibody, Unconjugated (orb500076) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CK10) Monoclonal Antibody, Unconjugated (orb500076) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Sample: Skin (Mouse) Lysate at 40 ug, Skin (Rat) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-CK10/Cytokeratin 10 (orb500076) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 63kD.
	WB analysis of Skin (Mouse) Lysate at 40 ug, Skin (Rat) Lysate at 40 ug using CK10 antibody
	IHC-P image of mouse skin tissue using CK10 antibody
	IHC-P image of rat skin tissue using CK10 antibody

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