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IHC-P of mouse embryo tissue (dilution at:1:200) using AXL antibody |
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Sample: Lovo (Human) Cell Lysate at 40 ug, K562 (Human) Cell Lysate at 40 ug, Primary: Anti-AXL (orb4399) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 95 kD, Observed band size: 95 kD. |
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Sample: MKN45 (Human) Cell Lysate at 30 ug, SW480 (Human) Cell Lysate at 30 ug, FHC (Human) Cell Lysate at 30 ug, Primary: Anti-AXL (orb4399) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14012098 kD, Observed band size: 120 kD. |
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Sample: Testis (Mouse) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-AXL (orb4399) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 140/120/65 kD, Observed band size: 120 kD. |
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Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated (orb4399) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated (orb4399) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Western blot analysis of Human Lovo(lane 1), K562(lane 2) Cell Lysate using AXL antibody |