AXL Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB4399
Artikelname: AXL Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB4399
Hersteller Artikelnummer: orb4399
Alternativnummer: BYT-ORB4399-50,BYT-ORB4399-100,BYT-ORB4399-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: KLH conjugated synthetic peptide derived from human AXL (151-250/894aa)
Konjugation: Unconjugated
Alternative Synonym: ARK, AXL3, JTK11, Tyro7, UFO, UFO_HUMAN, AXL, AXL oncogene, 2.7.10.1, UFO_MOUSE, Adhesion-related kinase,
AXL Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 95 kDa
UniProt: P30530
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: AXL
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
IHC-P of mouse embryo tissue (dilution at:1:200) using AXL antibody
Sample: Lovo (Human) Cell Lysate at 40 ug, K562 (Human) Cell Lysate at 40 ug, Primary: Anti-AXL (orb4399) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 95 kD, Observed band size: 95 kD.
Sample: MKN45 (Human) Cell Lysate at 30 ug, SW480 (Human) Cell Lysate at 30 ug, FHC (Human) Cell Lysate at 30 ug, Primary: Anti-AXL (orb4399) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14012098 kD, Observed band size: 120 kD.
Sample: Testis (Mouse) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-AXL (orb4399) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 140/120/65 kD, Observed band size: 120 kD.
Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated (orb4399) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated (orb4399) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Western blot analysis of Human Lovo(lane 1), K562(lane 2) Cell Lysate using AXL antibody