Immunohistochemical staining of human lung carcinoma tissue using CPNE1 antibody.
Western blot analysis of mouse spleen cell lysate using CPNE1 antibody.
Protein: spleen (mouse) lysate at 40 ug, Primary: rabbit Anti-CPNE1 (orb182711) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 59 kD, Observed band size: 59 kD.
Tissue/Cell: human liver carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated (orb182711) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated (orb182711) 1:400, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated (orb182711) 1:400, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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