CPNE1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB182711
Artikelname: CPNE1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB182711
Hersteller Artikelnummer: orb182711
Alternativnummer: BYT-ORB182711-50,BYT-ORB182711-100,BYT-ORB182711-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from human CPNE1 (1-120/537aa)
Konjugation: Unconjugated
Alternative Synonym: COPN1, CPN1, 1810028N16Rik, mKIAA4108, CPNE1_HUMAN, CPNE1, Chromobindin 17, Copine I, CPNE1_MOUSE,
CPNE1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 59 kDa
UniProt: Q99829
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: CPNE1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of human lung carcinoma tissue using CPNE1 antibody.
Western blot analysis of mouse spleen cell lysate using CPNE1 antibody.
Protein: spleen (mouse) lysate at 40 ug, Primary: rabbit Anti-CPNE1 (orb182711) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 59 kD, Observed band size: 59 kD.
Tissue/Cell: human liver carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated (orb182711) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated (orb182711) 1:400, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated (orb182711) 1:400, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.