COX4I1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256931
Article Name: COX4I1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1256931
Supplier Catalog Number: orb1256931
Alternative Catalog Number: BYT-ORB1256931-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-169 of human COX IV (NP_001852.1).
Conjugation: Unconjugated
Alternative Names: COX4I1, COX4, COXIV, MGC72016, COX4-1
COX4I1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 17kDa
UniProt: P13073
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: COX4I1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using COX IV Antibody (orb1256931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat spleen using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using COX IV antibody (orb1256931). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 22Rv1 cells, using 3 ug COX IV antibody (orb1256931). Western blot was performed from the immunoprecipitate using COX IV antibody (orb1256931) at a dilution of 1:1000.