COX4I1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1256931
Artikelname: COX4I1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1256931
Hersteller Artikelnummer: orb1256931
Alternativnummer: BYT-ORB1256931-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-169 of human COX IV (NP_001852.1).
Konjugation: Unconjugated
Alternative Synonym: COX4I1, COX4, COXIV, MGC72016, COX4-1
COX4I1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 17kDa
UniProt: P13073
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: COX4I1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using COX IV Antibody (orb1256931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat spleen using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using COX IV antibody (orb1256931). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 22Rv1 cells, using 3 ug COX IV antibody (orb1256931). Western blot was performed from the immunoprecipitate using COX IV antibody (orb1256931) at a dilution of 1:1000.