BRDT Antibody - N-terminal region
Catalog Number:
ASB-ARP38071_P050
- Images (4)
| Article Name: | BRDT Antibody - N-terminal region |
| Biozol Catalog Number: | ASB-ARP38071_P050 |
| Supplier Catalog Number: | ARP38071_P050 |
| Alternative Catalog Number: | ASB-ARP38071_P050-25UL,ASB-ARP38071_P050-100UL |
| Manufacturer: | Aviva |
| Host: | Rabbit |
| Category: | Proteine/Peptide |
| Application: | IHC |
| Species Reactivity: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human BRDT |
| Alternative Names: | CT9, BRD6, SPGF21 |
| BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Two transcript variants encoding the same protein have been found for this gene. |
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Rabbit Anti-BRDT antibody Catalog Number: ARP38071 Formalin Fixed Paraffin Embedded Tissue: Human Testis Primary antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20x Exposure Time: 0.5-2.0sec |
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WB Suggested Anti-BRDT Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: MCF7 cell lysate |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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BRDT antibody - N-terminal region (ARP38071_P050) in Human Testis using Immunohistochemistry |
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