BRDT Antibody - N-terminal region

Artikelnummer: ASB-ARP38071_P050
Artikelname: BRDT Antibody - N-terminal region
Artikelnummer: ASB-ARP38071_P050
Hersteller Artikelnummer: ARP38071_P050
Alternativnummer: ASB-ARP38071_P050-25UL,ASB-ARP38071_P050-100UL
Hersteller: Aviva
Wirt: Rabbit
Kategorie: Proteine/Peptide
Applikation: IHC
Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BRDT
Alternative Synonym: CT9, BRD6, SPGF21
BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Two transcript variants encoding the same protein have been found for this gene.
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 108kDa
NCBI: 676
UniProt: Q58F21
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Rabbit Anti-BRDT antibody
Catalog Number: ARP38071
Formalin Fixed Paraffin Embedded Tissue: Human Testis
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec
WB Suggested Anti-BRDT Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
BRDT antibody - N-terminal region (ARP38071_P050) in Human Testis using Immunohistochemistry