NR3C2 Antibody - N-terminal region

Catalog Number: ASB-ARP31008_P050
Article Name: NR3C2 Antibody - N-terminal region
Biozol Catalog Number: ASB-ARP31008_P050
Supplier Catalog Number: ARP31008_P050
Alternative Catalog Number: ASB-ARP31008_P050-25UL,ASB-ARP31008_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NR3C2
Alternative Names: MR, MCR, MLR, NR3C2VIT
NR3C2 is a receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. It binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase io
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 107kDa
NCBI: 4306
UniProt: P08235
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
WB Suggested Anti-NR3C2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HT1080 cell lysate
Host: Rabbit
Target Name: NR3C2
Sample Tissue: Human 293T Whole Cell
Antibody Dilution: 0.5ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
NR3C2 antibody - N-terminal region (ARP31008_P050) in Human HT1080 using Western Blot