Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human NR3C2
Alternative Synonym:
MR, MCR, MLR, NR3C2VIT
NR3C2 is a receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. It binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase io
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
NR3C2 antibody - N-terminal region (ARP31008_P050) in Human HT1080 using Western Blot
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