SLC25A13/Citrin Rabbit mAb, Unconjugated, Monoclonal

Article Name:	SLC25A13/Citrin Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A23756
Supplier Catalog Number:	A23756
Alternative Catalog Number:	ABB-A23756-100UL,ABB-A23756-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	SLC25A13, ARALAR2, CITRIN, CTLN2, solute carrier family 25 member 13, SLC25A13/Citrin

This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene.

Clonality:	Monoclonal
Molecular Weight:	74kDa
NCBI:	10165
UniProt:	Q9UJS0
Purity:	Affinity purification
Target:	SLC25A13

Application Dilute:	WB,1:2000 - 1:8000\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. Shipping: Ice Bag

	Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): MOLT-4 Exposure time: 10s.
	Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s.
	Immunohistochemistry analysis of paraffin-embeddedHuman colon carcinoma tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman liver tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman liver cancer tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse kidney tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat kidney tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat liver tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.