SLC25A13/Citrin Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A23756
Artikelname: SLC25A13/Citrin Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A23756
Hersteller Artikelnummer: A23756
Alternativnummer: ABB-A23756-100UL,ABB-A23756-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: SLC25A13, ARALAR2, CITRIN, CTLN2, solute carrier family 25 member 13, SLC25A13/Citrin
This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene.
Klonalität: Monoclonal
Molekulargewicht: 74kDa
NCBI: 10165
UniProt: Q9UJS0
Reinheit: Affinity purification
Target-Kategorie: SLC25A13
Application Verdünnung: WB,1:2000 - 1:8000|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. Shipping: Ice Bag
Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): MOLT-4
Exposure time: 10s.
Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embeddedHuman colon carcinoma tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedHuman liver tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedHuman liver cancer tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedMouse kidney tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedRat kidney tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedRat liver tissue usingSLC25A13/Citrin Rabbit mAb(A23756) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.