RSK2 / RPS6KA3 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	RSK2 / RPS6KA3 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A20868
Supplier Catalog Number:	A20868
Alternative Catalog Number:	ABB-A20868-100UL,ABB-A20868-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	CLS, RSK, HU-3, RSK2, MRX19, ISPK-1, XLID19, p90-RSK2, pp90RSK2, MAPKAPK1B, S6K-alpha3, RSK2 / RPS6KA3

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).

Clonality:	Monoclonal
Molecular Weight:	84kDa
NCBI:	6197
UniProt:	P51812
Purity:	Affinity purification
Target:	RPS6KA3

Application Dilute:

WB,1:100 - 1:500|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Application Notes:

Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Serine threonine kinases,mTOR Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Cell Cycle,Cell Cycle Control-G2 M DNA Damage Checkpoint,Microtubules,Immunology Inflammation,Neuroscience. Shipping: Ice Bag

	Western blot analysis of various lysates using RSK2 / RPS6KA3 Rabbit mAb (A20868) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of lysates from NIH/3T3 cells, using RSK2 / RPS6KA3 Rabbit mAb (A20868) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry analysis of paraffin-embeddedHuman breast cancer tissue usingRSK2 / RPS6KA3 Rabbit mAb(A20868) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman liver tissue usingRSK2 / RPS6KA3 Rabbit mAb(A20868) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat intestine tissue usingRSK2 / RPS6KA3 Rabbit mAb(A20868) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse lung tissue usingRSK2 / RPS6KA3 Rabbit mAb(A20868) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse intestin tissue usingRSK2 / RPS6KA3 Rabbit mAb(A20868) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat liver tissue usingRSK2 / RPS6KA3 Rabbit mAb(A20868) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.