MYH4 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A15293
- Images (7)
| Article Name: | MYH4 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A15293 |
| Supplier Catalog Number: | A15293 |
| Alternative Catalog Number: | ABB-A15293-100UL,ABB-A15293-20UL,ABB-A15293-500UL,ABB-A15293-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | MYH2B, MyHC-2B, MyHC-IIb, MYH4 |
| Enables double-stranded RNA binding activity. Involved in muscle contraction. Located in myofibril. |
| Application Dilute: | WB,1:500 - 1:5000|IF-P,1:50 - 1:200|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Motor Proteins. Shipping: Ice Bag |
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Western blot analysis of lysates from Mouse skeletal muscle, using MYH4 Rabbit pAb (A15293) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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Western blot analysis of various lysates, using MYH4 Rabbit pAb (A15293) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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Immunohistochemistry analysis of paraffin-embedded Mouse heart using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse skeletal muscle using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat heart using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat skeletal muscle using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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