MYH4 Rabbit pAb, Unconjugated, Polyclonal

Artikelnummer: ABB-A15293
Artikelname: MYH4 Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer: ABB-A15293
Hersteller Artikelnummer: A15293
Alternativnummer: ABB-A15293-100UL,ABB-A15293-20UL,ABB-A15293-500UL,ABB-A15293-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: MYH2B, MyHC-2B, MyHC-IIb, MYH4
Enables double-stranded RNA binding activity. Involved in muscle contraction. Located in myofibril.
Klonalität: Polyclonal
Molekulargewicht: 223 kDa
NCBI: 4622
UniProt: Q9Y623
Reinheit: Affinity purification
Sequenz: ETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVES
Target-Kategorie: MYH4
Application Verdünnung: WB,1:500 - 1:5000|IF-P,1:50 - 1:200|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Motor Proteins. Shipping: Ice Bag
Western blot analysis of lysates from Mouse skeletal muscle, using MYH4 Rabbit pAb (A15293) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of various lysates, using MYH4 Rabbit pAb (A15293) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse skeletal muscle using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat heart using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat skeletal muscle using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using MYH4 Rabbit pAb (A15293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.