RAB5A Rabbit pAb, Unconjugated, Polyclonal

Article Name:	RAB5A Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number:	ABB-A1180
Supplier Catalog Number:	A1180
Alternative Catalog Number:	ABB-A1180-20UL,ABB-A1180-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	RAB5, RAB5A

Enables GDP binding activity, GTP binding activity, and GTPase activity. Involved in several processes, including amyloid-beta clearance by transcytosis, early endosome to late endosome transport, and regulation of exocytosis. Located in several cellular components, including cytoplasmic side of early endosome membrane, nucleoplasm, and terminal bouton.

Clonality:	Polyclonal
Molecular Weight:	24kDa
NCBI:	5868
UniProt:	P20339
Purity:	Affinity purification
Target:	RAB5A

Application Dilute:	WB,1:500 - 1:1000\|IHC-P,1:50 - 1:200\|IF/ICC,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Neuroscience, Cell Type Marker,Neurodegenerative Diseases,Neuron marker,Synapse marker. Shipping: Ice Bag

	Western blot analysis of various lysates, using RAB5A Rabbit pAb (A1180) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry analysis of paraffin-embedded Human liver cancer using RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human liver using RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spleen using RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat spleen using RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunofluorescence analysis of C6 cells using RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.