PIAS1+PIAS2 Rabbit mAb, Unconjugated, Monoclonal

Artikelname:	PIAS1+PIAS2 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A9670
Hersteller Artikelnummer:	A9670
Alternativnummer:	ABB-A9670-100UL,ABB-A9670-20UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	DDXBP1, GBP, GU/RH-II, ZMIZ3, PIAS1+PIAS2

This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Klonalität:	Monoclonal
Molekulargewicht:	76kDa
NCBI:	8554
UniProt:	O75925
Reinheit:	Affinity purification
Target-Kategorie:	PIAS1/PIAS2

Application Verdünnung:	WB,1:500 - 1:2000\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Human,Mouse,Rat. Shipping: Ice Bag

	Western blot analysis of various lysates using PIAS1+PIAS2 Rabbit mAb (A9670) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry analysis of paraffin-embeddedHuman thyroid tissue usingPIAS1+PIAS2 Rabbit mAb(A9670) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman liver tissue usingPIAS1+PIAS2 Rabbit mAb(A9670) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingPIAS1+PIAS2 Rabbit mAb(A9670) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse heart tissue usingPIAS1+PIAS2 Rabbit mAb(A9670) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingPIAS1+PIAS2 Rabbit mAb(A9670) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman colon tissue usingPIAS1+PIAS2 Rabbit mAb(A9670) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat testis tissue usingPIAS1+PIAS2 Rabbit mAb(A9670) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.