[KD Validated] MRPL20 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:
ABB-A26199
- Bilder (8)
| Artikelname: | [KD Validated] MRPL20 Rabbit mAb, Unconjugated, Monoclonal |
| Artikelnummer: | ABB-A26199 |
| Hersteller Artikelnummer: | A26199 |
| Alternativnummer: | ABB-A26199-500UL,ABB-A26199-20UL,ABB-A26199-1000UL,ABB-A26199-100UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | L20mt, bL20m, MRP-L20 |
| Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Application Verdünnung: | WB,1:2000 - 1:13000|IHC-P,1:200 - 1:800|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Tags & Cell Markers Subcellular Markers Organelles MitochondriaEpigenetics and Nuclear Signaling DNA / RNA Translation RibosomeEpigenetics and Nuclear Signaling DNA / RNA Translation Mito. TranslationMetabolism Pathways and Processes Mitochondrial Metabolism Mitochondrial translationMetabolism Pathways and Processes Mitochondrial Metabolism Mitochondrial markers. Shipping: Ice Bag |
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Western blot analysis of various lysates using [KD Validated] MRPL20 Rabbit mAb (A26199) at 1:13000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30 s. |
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Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KD Validated] MRPL20 Rabbit mAb (A26199) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Western blot analysis of lysates from wild type (WT) and MRPL20 knockdown (KD) HeLa cells using [KD Validated] MRPL20 Rabbit mAb (A26199) at 1:2000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] MRPL20 Rabbit mAb (A26199) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] MRPL20 Rabbit mAb (A26199) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] MRPL20 Rabbit mAb (A26199) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] MRPL20 Rabbit mAb (A26199) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KD Validated] MRPL20 Rabbit mAb (A26199) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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