Our partner Enzo Life Sciences is a life sciences and biotechnology company, focusing on harnessing biological process to develop research tools, diagnostics and therapeutics, and serves as a provider of test services, including exotic tests, to the medical community. With comprehensive expertise in experimental settings, Enzo' Senior Application Scientist Dr. Hartmut Pohl provides detailed advise on how often an ELISA measurement should be repeated. &n...

The SPHERO™ Calibration Particles are versatile, stable, economical and convenient to use. These particles contain a mixture of fluorochrome which are spectrally similar to many of the fluorochromes used in flow cytometry. As a result, they are used for routine alignment, day-to-day performance verification and long term performance tr...

        Described is the Atlas Antibodies standard immunohistochemistry protocol optimized for Triple A Polyclonals and PrecisA Monoclonals.   Deparaffinization Paraffin sections of 4 µm thickness are baked overnight at 50°C. Prior to immunostaining, depa...

Tissue Sectioning, Deparaffinization, and Rehydration Section paraffin blocks into 4 micron sections with microtome and place on charged microscope slides (Fisher, ProbeOn, Cat. #22230900). Heat slides in a tissue-drying oven for 45 minutes at 60°C. Wash slides in 3 changes of xylene for 5 minutes each at room temperature. Wash slides in 3 changes of 100% alcohol for 3 minutes each at room temperature. Wash slides in 2 changes of 95% alcohol for 3 minutes each at room temperature.

After a few intense days of meticulous tissue washes and antibody incubations my staining is finally ready. I am staring at it down the microscope. I enjoy what I see: a dark brown color exactly where I expect to see it! Am I good or what? I give myself a figurative pat on the back and an imaginary high five. Excited and proud, I call my supervisor who glares intently at it and...

Plasma membrane (PM) proteins play a critical role in a variety of physiological and pathological processes. Signal transduction, molecular transport and cell-cell interactions are all mediated by PM proteins. PM proteins include a variety of important proteins such as neurotransmitter receptors, G-proteins, c...

The main problems currently associated with systemic gene vector administration (gene therapy) include biodistribution of gene vector throughout the body, the lack of specificity towards a pathological site (bioavailability at the target site), the necessity of a large dose to achieve high local concentration, non-specific toxicity, inactivation of vectors due to undesired interactions with components of the in vivo mi...

MBL is a leading life science company focused on providing high quality, innovative, solutions-based products for both life science research and clinical diagnostics. MBL develops, manufactures, and markets a broad portfolio of monoclonal and polyclonal antibodies, MHC tetramers, fluorescent and recombinant proteins and ELISA ki...

Our partner GeneTex supports researchers with a comprehensive selection of troubleshooting tips for many issues which can occur in a western blot (WB) experiment. Download also the

Biorbyt develops biochemicals, antibodies and immunoassays of the highest quality. For Western Blot, Biorbyt provides a wide range of matching antibodies blocking peptides.   Non-Specific Binding & Blocking Peptides   Non-Specific Binding On West...