NCX1 (Na+/Ca2+ Exchange Protein 1, CNC, DKFZp779F0871, MGC119581, Na+/Ca2+ Exchanger, NCX, Solute Carrier Family 8 Member 1, SLC8A1), IgM, Clone: [9A71], Mouse, Monoclonal
Biozol Catalog Number:
USB-N0560-21
Supplier Catalog Number:
N0560-21
Alternative Catalog Number:
USB-N0560-21-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
ELISA, FC, IF, IHC, IP, WB
Immunogen:
Purified canine cardiac sodium/calcium exchanger
The sodium/calcium exchanger of cardiac sarcolemma rapidly transports calcium during excitation-contraction coupling and is the dominant myocardial calcium efflux mechanism. The sodium/calcium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium against its electrochemical gradient in a 3 sodium : 1 calcium exchange. Sodium/calcium exchange activity is present in excitable cells and in non-excitable cells. Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence/Immunocytochemistry, Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:100 Immunofluorescence (IC): 1:100-1:200 Immunohistochemistry: 1:20-1:50 Immunohistochemistry (Paraffin): 1:100 Immunohistochemistry (Frozen): 1:100 Immunoprecipitation: 1:10-1:500 Western Blot: 1:1000. Detects a 120kD protein representing the sodium/calcium exchanger from guinea pig cardiac extract. The bands seen at 70kD and 160kD represent a proteolytic fragment and non-reduced exchanger respectively. T-tubular membrane. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[9A71]
Isotype:
IgM
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
* VAT and and shipping costs not included. Errors and price changes excepted
