Synthetic peptide consisting of 18aa sequence within the N-terminus of mature rat NCX1 conjugated to KLH. Species sequence homology: mouse-100%, feline-77%, human, rabbit, monkey, bovine-72%, chicken-66%.
Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein. NCX1 (rat 971 aa, human 970aa, mouse 970aa) is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling, but is also present in most other tissues. Alternative spicing of NCX1/NACA1 produces numerous tissue specific isoforms (heart NACA1, Kidney NACA2, -3, and -7, brain NACA4-6). NCX2 (rat 921aa) is restricted to brain and skeletal muscle. NCX3 (rat 927aa) shares 73-75% with NCX1 and NCX2. NCX3 is also restricted to brain and muscle. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. ELISA: 0.5-1ug/ml. Control peptide N0560-20B can be used to coat ELISA plates at 1ug/ml. Immunohistochemistry (PFFE): 5-20ug/ml. 4% paraformaldehyde. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified over the peptide-Sepharose column
Form:
Supplied as a lyophilized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
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