Synthetic peptide corresponding to human monoglyceride lipase.
Endocannabinoids, such as arachidonyl ethanolamide (AEA) and 2-Arachidonyl glycerol (2-AG), function as short-range modulators of cell and synaptic activity. Monoacylglycerol lipase (MAGL) hydrolyzes 2-AG to terminate its biological actions and works consecutively with hormone-sensitive lipase (HSL) to mobilize fatty acids from the triglyceride stores of adipocytes. MAGL has a molecular weight of ~33kD and exhibits a high degree of homology among human, mouse, and rat at the amino acid level. MAGL is expressed in a variety of tissues such as kidney, spleen, heart, liver, testis, stomach, brain, lung, and adrenal gland, with most abundant expression in skeletal muscle and adipose tissue. This suggests a role of MAGL in monoglyceride hydrolysis in diverse tissues. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:300, Band at 35kD Immunohistochemistry (formalin-fixed parrafin-embedded tissue): 1:150 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
