Monocytes/Macrophages, IgG2c, Clone: [ER-HR3], Rat, Monoclonal
Biozol Catalog Number:
USB-M4520-15A
Supplier Catalog Number:
M4520-15A
Alternative Catalog Number:
USB-M4520-15A-100
Manufacturer:
US Biological
Host:
Rat
Category:
Antikörper
Application:
FC, IHC
Immunogen:
Adherent F1 (CBAxBL) bone marrow stromal cells.
Whereas all other leukocytes are ER-HR3 negative, up to 70% of circulating monocytes stain positive with this monoclonal antibody. Furthermore, about 30% of bone marrow cells express the antigen. Other ER-HR3 positive macrophages can be found in the splenic red pulp, in the mesenteric lymphoid paracortex, interfollicular areas of Peyers patches and bone marrow. Few positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen. The monoclonal antibody can be used to identify and localize a very distinct mature tissue macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Immunohistochemistry (frozen and paraffin): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[ER-HR3]
Isotype:
IgG2c
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
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