MaxLight(TM)490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation, Western Blot, Flow Cytometry and Radioimmunoassay. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: frozen sections Immunohistochemistry (Paraffin embedded tissue (Bouin fixation)): requires pretreatment with trypsin or pronase Flow Cytometry: 10ul labels 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[2Q1782 ED1]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight(TM)490.
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