Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation, Western Blot and Radioimmunoassay. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: frozen sections Immunohistochemistry (Paraffin embedded tissue (Bouin fixation)): requires pretreatment with trypsin or pronase Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[2Q1782 ED1]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
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