MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Pr
Catalog Number:
USB-M2363
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| Article Name: | MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Pr |
| Biozol Catalog Number: | USB-M2363 |
| Supplier Catalog Number: | M2363 |
| Alternative Catalog Number: | USB-M2363-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IP, WB |
| Immunogen: | KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. |
| Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 0.1-1ug/ml detects pMAPK in RIPA lysates from EGF-stimulated human A431 carcinoma cells. Non-stimulated A431 and EGF-stimulated A431 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-pMAPK (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Use 1-10ug per immunoprecipitation. Optimal dilutions to be determined by the researcher. Positive Antigen Control: EGF-stimulated A431 lysate. Use 20ug per lane for minigels. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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