MAPKAP Kinase 2 (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2), Rabbit
Biozol Catalog Number:
USB-M2355-10A
Supplier Catalog Number:
M2355-10A
Alternative Catalog Number:
USB-M2355-10A-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
FC, IP, WB
Immunogen:
Synthetic peptide in the C-terminal region of human MAPKAPK-2. Species sequence homology: hamster
In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been illustrated that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four of the residues-Thr25, Thr222, Ser272 and Thr334-are phosphorylated by p38 in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 seems to be essential for the activity of MAPKAPK-2 (2). Thr25 is also phosphorylated by p42 MAPK in vitro and is not required for the activation of MAPKAPK-2 (2). Applications: Suitable for use in Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
