Two synthetic peptides corresponding to different regions of the MAP-2 gene product (KLH). Species sequence homology: human (NP_002365, NCBI) and mouse (P20357, NCBI) 100%.
Human MAP-2 is a 199.296 kD protein (1827aa) expressed in neurons of the PNS and CNS, where it serves as a major component of the neuronal cytoskeleton. MAP-2 contributes to structural integrity and cell shape. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells Western Blot: 1:2000-1:5000 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.