MAP Kinase, p38 (MAPK14, Mitogen-activated Protein Kinase 14, CSBP), Rabbit
Biozol Catalog Number:
USB-M2352
Supplier Catalog Number:
M2352
Alternative Catalog Number:
USB-M2352-200
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
FC, IF, IHC, WB
Immunogen:
Synthetic peptide corresponding to sequence of human p38 MAPK. Species Sequence Homology: chicken
P38 is the newest member of the MAP kinase family. It is a reactivating kinase. It is activated in response to inflammatory cytokines, endotoxins, and osmotic stress. p38 MAP kinase (MAPK), also called RK or CSBP, is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress. Four isoforms of p38 MAP kinase, alpha, beta, gamma and delta, have been identified so far. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors. MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max and MEF2. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membranes with diluted antibody, 1X TBS, 5% BSA, 0.1% Tween-20 at 4C with gentle shaking overnight.. Immunohistochemistry (Paraffin): 1:100. Antigen retrieval with citrate buffer required. Immunofluorescence (IC): 1:50 Flow Cytometry: 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
