Human b1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. Specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.
The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit non-convalently associated with the common b1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. This antibody recognizes epitopes in the 355-425 region of the common b1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli. Applications: Suitable for use in ELISA, Western Blot, FACS and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Optimal dilution determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
