Integrin beta1, Activating (MaxLight 650), IgG1, Clone: [2Q849 P4G11], Mouse, Monoclonal
Catalog Number:
USB-I7661-38-ML650
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| Article Name: | Integrin beta1, Activating (MaxLight 650), IgG1, Clone: [2Q849 P4G11], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-I7661-38-ML650 |
| Supplier Catalog Number: | I7661-38-ML650 |
| Alternative Catalog Number: | USB-I7661-38-ML650-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | FC, FLISA, IHC, IP |
| Immunogen: | Human IL-1b activated endothelial cells. |
| MaxLight(TM)650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor(TM)647, DyLight(TM)649, Cy5(TM) and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm), Emission (676nm), Extinction Coefficient 250,000. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and FLISA. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 2ug/ml (use on numerous cell lines as long as Ca2+ is present), Immunoprecipitation: 5ug/400ul lysate (0.5% triton PBS plus Ca++) FACS: 2-5ug/10e6 cells, in the presence of Ca++. Stimulation of cells: 2-10ug/ml per 10e6 cells. Binding buffer must contain 1mM Ca++ Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, Qin et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers. Optimal dilution determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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