Integrin beta1, Activating (Biotin), IgG1, Clone: [2Q849 P4G11], Mouse, Monoclonal
Catalog Number:
USB-I7661-38-BIOTIN
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| Article Name: | Integrin beta1, Activating (Biotin), IgG1, Clone: [2Q849 P4G11], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-I7661-38-BIOTIN |
| Supplier Catalog Number: | I7661-38-Biotin |
| Alternative Catalog Number: | USB-I7661-38-BIOTIN-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, FC, IHC, IP |
| Immunogen: | Human IL-1b activated endothelial cells. |
| Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 2ug/ml (use on numerous cell lines as long as Ca2+ is present), Immunoprecipitation: 5ug/400ul lysate (0.5% triton PBS plus Ca++) FACS: 2-5ug/10e6 cells, in the presence of Ca++. Stimulation of cells: 2-10ug/ml per 10e6 cells. Binding buffer must contain 1mM Ca++ Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, Qin et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers. Optimal dilution determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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