Immunization of beta6 knockout mice with wild type mouse keratinocytes.
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumors. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alphaV beta6 is upregulated in cancers and during tissue remodelling but is absent from resting adult tissues. Integrin alphaV beta6 promotes invasion and correlates with poor survival and therefore makes a promising therapeutic target. Applications: Suitable for use in Immunoprecipitation and Function Blocking. Not suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunoprecipitation: Effective. Protein G or rabbit anti-mouse IgG recommended for capture. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
