Human alpha 1 integrin sub-unit forms the VLA-1 heterodimer in association with the beta 1 integrin. VLA-1 is a receptor for collagen and laminin, and is expressed by chronically activated T cells, melanoma cells and smooth muscle cells. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:25-1:50. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Recommended Secondary Reagents: F(ab)2 rabbit anti mouse IgG:RPE I1904-66A: IgG, F(ab)2 (FITC) Pab Rb xMo I1904-65W: IgG, F(ab)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab)2 X-adsorbed (HRP) Pab Rb xMo Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
