Granzyme A, Recombinant, Human, His-Tag
Catalog Number:
USB-169686
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| Article Name: | Granzyme A, Recombinant, Human, His-Tag |
| Biozol Catalog Number: | USB-169686 |
| Supplier Catalog Number: | 169686 |
| Alternative Catalog Number: | USB-169686-10 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| Granzyme A is a member of the granzyme family. Granzyme A is the most abundant serine protease in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A has a specific function in CTL and NK cells. It induces caspase-independent cell death when introduced into target cells by perforin. Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1-26), a propeptide (residues 27-28) and a mature chain (residues 29-262). Recombinant protein corresponding to Cys26-Val262 of human Granzyme A with a C-terminal 10 His tag expressed in NSO cell line. Swiss/UniProt Accession: P12544. Synonyms: GZMA, Granzyme-1, CTL Tryptase, Cytotoxic T-lymphocyte-associated Serine Esterase 3, CTLA3, Cytotoxic T-lymphocyte Proteinase 1, Fragmentin-1, Hanukkah Factor, H Factor, HF, HFSP Structure: Pro form Molecular Weight: 252 amino acid recombinant protein has a predicted molecular mass of approximately 28kD. The protein migrates at about 35kD in DTT-reducing conditions and about 55kD in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid is Cys. Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >5,000 pmol/min/g, as measured under the described conditions. Endotoxin: 1.0 EU per 1ug of the protein by the LAL method. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute at 100ug/ml in sterile 25 mM HEPES, 150mM NaCl and 10mM CaCl2, pH 7.5. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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