Myosin Light Chain 2, Regulatory, Cardiac (MYL2) Recombinant, Human
Biozol Catalog Number:
USB-155995
Supplier Catalog Number:
155995
Alternative Catalog Number:
USB-155995-10,USB-155995-50,USB-155995-200
Manufacturer:
US Biological
Category:
Molekularbiologie
Recombinant protein corresponding to Ala2-Ile159 with an N-Terminal His Tag from human MYL2, expressed in E. coli (P10916) Amino Acid Sequence: APKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHII Predicted Isoelectric Point: 5.5 Predicted Molecular Weight: 19.1kD Accurate Molecular Mass: 21kD as determined by SDS-PAGE under reducing conditions Subcellular Location: Cytoplasm Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from 100mM sodium bicarbonate, 500mM sodium chloride, pH 8.3, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin 300. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex
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