Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) Recombinant, Human
Biozol Catalog Number:
USB-155991
Supplier Catalog Number:
155991
Alternative Catalog Number:
USB-155991-10,USB-155991-50,USB-155991-200
Manufacturer:
US Biological
Category:
Molekularbiologie
Recombinant protein corresponding to Phe3-Ala142 with N-Terminal His Tag from human MYL1, expressed in E. coli Swiss/UniProt Accession: P05976. Amino Acid Sequence: FSADQIAE FKEAFLLFDR TGDSKITLSQ VGDVLRALGT NPTNAEVRKV LGNPSNEELN AKKIEFEQFL PMMQAISNNK DQATYEDFVE GLRVFDKEGN GTVMGAELRH VLATLGEKMK EEEVEALMAG QEDSNGCINY EA Predicted Isoelectric Point: 4.9 Predicted Molecular Weight: 16.8kD Accurate Molecular Mass: 17kD as determined by SDS-PAGE under reducing conditions Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride pH 8.0, 0.01% sarcosyl, 5% Trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.
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