Recombinant protein corresponding to aa22-119 with an N-terminal His-Tag, from mouse b2M expressed in E. coli (P01887) Amino Acid Sequence: MGHHHHHHSGS-QKTPQIQVY SRHPPENGKP NILNCYVTQF HPPHIEIQML KNGKKIPKVE MSDMSFSKDW SFYILAHTEF TPTETDTYAC RVKHDSMAEP KTVYWDRDM Predicted Isoelectric Point: 6.1 Predicted Molecular Weight: 12.8kD Subcellular Location: Secreted Applications: Suitable for use as a positive control, Immunogen, SDS-PAGE and Western Blot. Other applications not tested Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from 100mM NaHCO3,, 500mM sodium chloride, pH 8.3, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile dH2O to a concentration of 0.1-1mg/ml. Do not vortex.
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