Source: Recombinant Mouse from E. coli Accession No: Q61490 Fragment: Trp28~Lys527 (Accession No: Q61490) Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WYT VNSAYGDTIV MPCRLDVPQN LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL SLSENYTLSI ANAKISDEKR FVCMLVTEDN VFEAPTLVKV FKQPSKPEIV NKAPFLETDQ LKKLGDCISR DSYPDGNITW YRNGKVLQPV EGEVAILFKK EIDPGTQLYT VTSSLEYKTT RSDIQMPFTC SVTYYGPSGQ KTIYSEQEIF DIYYPTEQVT IQVLPPKNAI KEGDNITLQC LGNGNPPPEE FMFYLPGQPE GIRSSNTYTL TDVRRNATGD YKCSLIDKRN MAASTTITVH YLDLSLNPSG EVTKQIGDTL PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIHWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV SAISIPEHDE ADDISDENRE KVNDQAK Epitope Tag: N-terminal Tags: His-tag and T7-tag Molecular Weight: 59.9kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
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