Source: Recombinant Canine from E. coli Accession No: O46634 Fragment: Cys208-His476 (Accession No: O46634) Sequence: MGHHHHHHSGSEF-CLG NGNPPPEEFL FYLPGQPEGI RSSNTYTLTDVRRNATGDYK CSLIDKKSMI ASTAITVHYL DLSLNPSGEV TKQIGDALPV SCTISASRNATVVWMKDNIR LRSSPSFSSL QYQDAGNYVC ETALQEVEGL KKRESLTLIV EGKPQIKMTKKTDPSGLSKT IICHVEGFPK PAIQWTITGS GSVINQTEES PYINGRYYST IINSPEENVTLTCTAENQLE RTVNSLNVSA ISIPEHDEAD EISDENREQV NHRATLIVGI VLRLLH Epitope Tag: N-terminal His-Tag Molecular Weight: 31.2kD Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
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