Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) BioAssay(TM) ELISA Kit (Human)
Catalog Number:
USB-152215
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| Article Name: | Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) BioAssay(TM) ELISA Kit (Human) |
| Biozol Catalog Number: | USB-152215 |
| Supplier Catalog Number: | 152215 |
| Alternative Catalog Number: | USB-152215-96 |
| Manufacturer: | US Biological |
| Category: | Kits/Assays |
| The Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ARPC2 in human tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.063ng/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *152215A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use *152215B: Standard, 2x1vial 152215C: Standard Diluent, 1x20ml *152215D: Detection Reagent A, 1x120ul *152215E: Detection Reagent B, 1x120ul 152215F: Assay Diluent A, 1x12ml 152215G: Assay Diluent B, 1x12ml 152215H: TMB Substrate, 1x9ml *152215J: Positive Control, xxng/ml, 1x1vial (Lyophilized) 152215K: Stop Solution, 1x6ml 152215L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (152215K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *152215A, *152215B, *152215D, *152215E and *152215J at -20C. After reconstitution of *152215J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution (Customer supplied). Read at 450nm immediately. |
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