Sandwich ELISA quantitative detection kit for Human Elafin/PI3 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Assay Range: 31.2-2000pg/ml Sensitivity: <10pg/ml Uniprot ID: P19957 Entrez Gene ID: 5266 Specificity: Natural and recombinant human Elafin/PI3. Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Kit Components: 143816A: Microtiter Strips, 1x96 wells *143816B: Elafin/PI3 Standard, 2x10ng 143816C: Anti-human Elafin/PI3 (Biotin), 1x130ul USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution) 143697: Sample Diluent Buffer, 1x30ml 143698: Antibody Diluent Buffer, 1x12ml 143699: ABC Diluent Buffer, 1x12ml 143700: TMB Color Developing Agent, 1x10ml 143701: TMB Stop Solution, 2N H2SO4, 1x10ml Storage and Stability: Store *143816B powder at 4C. Once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
