Sandwich ELISA quantitative detection kit for Human CD40 in cell culture supernates, serum, plasma ( eparin, EDTA), saliva, urine and human milk. CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells.1The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2.2The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.3 Assay Range: 15.6pg/ml-1000pg/ml Sensitivity: < 1 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P25942 Entrez Gene ID: 958 Kit Components: 96-well plate pre-coated with anti-CD40/TNFRSF5, Human Lyophilized recombinant CD40/TNFRSF5, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
