Free Thyroxine, FT4 BioAssay(TM) ELISA Kit (Human)
Catalog Number:
USB-058983
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| Article Name: | Free Thyroxine, FT4 BioAssay(TM) ELISA Kit (Human) |
| Biozol Catalog Number: | USB-058983 |
| Supplier Catalog Number: | 058983 |
| Alternative Catalog Number: | USB-058983-96 |
| Manufacturer: | US Biological |
| Category: | Kits/Assays |
| For the quantitative determination of human free Thyroxine in serum and plasma. Sensitivity: 1.08pmol/l Detection Range: 4-64pmol/l Specificity: This assay has high sensitivity and excellent specificity for detection of human FT4. No significant cross-reactivity or interference between human FT4 and analogues was observed. Assay Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of FT4 in the sample. The color development is stopped and the intensity of the color is measured. Kit Components: Microtiter Strips, 1x96 wells Standard (Freeze dried), 2xvial Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution: 1x10ml Storage and Stability: Store all kit components at 4C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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