Coagulation Factor X (F10) BioAssay(TM) ELISA Kit (Porcine)
Biozol Catalog Number:
USB-058947
Supplier Catalog Number:
058947
Alternative Catalog Number:
USB-058947-96
Manufacturer:
US Biological
Category:
Kits/Assays
For the quantitative determination of porcine coagulation factor X (F10) in serum, plasma, and tissue homogenates. Sensitivity: 0.027ug/ml Detection Range: 0.312-20ug/ml Specificity: This assay has high sensitivity and excellent specificity for detection of porcine F10. No significant cross-reactivity or interference between porcine F10 and analogues was observed. Assay Principle: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for F10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F10 present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for F10 is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F10 bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Microtiter Strips, 1x96 wells Standard (Freeze dried), 2xvial Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul HRP-conjugate Diluent, 1x20ml Sample Diluent, 2x20ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution: 1x10ml Storage and Stability: Store all kit components at 4C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
