The Cyclin Dependent Kinase 6 ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDK6 in human tissue homogenates and other biological fluids. Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDK6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CDK6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CDK6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of CDK6 in the sample is then determined by comparing the O.D. of the sample to the standard curve. Detection Range: 0.156-10ng/ml Sensitivity: 0.053ng/ml Kit Components: *024496A: Microtiter Plate, 1x96wells, Pre-coated, ready to use *024496B: Standard, 2x1vial 024496C: Standard Diluent, 1x20ml *024496D: Detection Reagent A (green), 1x120ul *024496E: Detection Reagent B (red), 1x120ul 024496F: Assay Diluent A, 2x, 1x6ml 024496G: Assay Diluent B, 2x, 1x6ml 024496H: TMB Substrate, 1x9ml 024496K: Stop Solution, 1x6ml 024496L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *024496A, *024496B, *024496D and *024496E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Materials Required But Not Supplied: 1. Microplate reader with 450 10nm filter. 2. Precision single or multi-channel pipettes and disposable tips. 3. Eppendorf Tubes for diluting samples. 4. Deionized or distilled water. 5. Absorbent paper for blotting the microtiter plate. 6. Container for Wash Solution
