Arginase (Arg) BioAssay(TM) ELISA Kit (Rat)
Catalog Number:
USB-023570
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| Article Name: | Arginase (Arg) BioAssay(TM) ELISA Kit (Rat) |
| Biozol Catalog Number: | USB-023570 |
| Supplier Catalog Number: | 023570 |
| Alternative Catalog Number: | USB-023570-96 |
| Manufacturer: | US Biological |
| Category: | Kits/Assays |
| The Rat Arginase (Arg) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of arginase in rat serum, plasma, tissue homogenates and other biological fluids. Detection Range: 1.56-100ng/ml Sensitivity: 0.61ng/ml Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Arginase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Arginase in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *023570A: Microtiter Plate, 96-well strip plate. Pre-coated, ready to use *023570B: Standard, 2x1vial 023570C: Standard Diluent, 1x20ml *023570D: Detection Reagent A (green), 1x120ul *023570E: Detection Reagent B (red), 1x120ul 023570F: Assay Diluent A, 2x, 1x6ml 023570G: Assay Diluent B, 2x, 1x6ml 023570H: TMB Substrate, 1x9ml 023570K: Stop Solution, 1x6ml 023570L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *023570A, *023570B, *023570D and *023570E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. |
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