Angiotensin I Converting Enzyme (ACE) BioAssay(TM) ELISA Kit (Human)
Catalog Number:
USB-023402
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| Article Name: | Angiotensin I Converting Enzyme (ACE) BioAssay(TM) ELISA Kit (Human) |
| Biozol Catalog Number: | USB-023402 |
| Supplier Catalog Number: | 023402 |
| Alternative Catalog Number: | USB-023402-96 |
| Manufacturer: | US Biological |
| Category: | Kits/Assays |
| The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACE in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.39 -25ng/ml Sensitivity: 0.17ng/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *023402A: Microtiter Plate, 1x96wells, Pre-coated, ready to use *023402B: Standard, 2x1vial 023402C: Standard Diluent, 1x20ml *023402D: Detection Reagent A, 1x120ul *023402E: Detection Reagent B, 1x120ul 023402F: Assay Diluent A, 2x, 1x6ml 023402G: Assay Diluent B, 2x, 1x6ml 023402H: TMB Substrate, 1x9ml 023402K: Stop Solution, 1x6ml 023402L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (023402K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023402A, *023402B, *023402D and *023402E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul 023402H: TMB Substrate. Incubate 15-25 minutes at 37C. 7. Add 50ul 023402K: Stop Solution. Read at 450nm immediately. |
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